Journal: The Journal of Biological Chemistry

Article Title: Peroxiredoxin IV plays a critical role in cancer cell growth and radioresistance through the activation of the Akt/GSK3 signaling pathways

doi: 10.1016/j.jbc.2022.102123

Figure Lengend Snippet: Examination of Prx4 protein levels in patient specimens of prostate cancer by tissue microarray or established prostate cancer cell lines by Western blotting. A , tissue microarray slides and representative images of anti-Prx4 staining of prostate normal and adenocarcinoma. Positive anti-Prx4 staining shows in dark brown and counter staining for nuclear shows light blue . The scale bars represent 5 mm (whole slide), 500 μm (individual tumor), and 100 μm (zoomed in). B and C , anti-Prx4 staining intensity were quantitatively scored by board-certified pathologist and data were compared between prostate normal (n = 50) and adenocarcinoma (n = 66) ( B ), as well as between prostate normal and cancer with different levels of Gleason grading ( C ). D , examination of Prx4 levels in prostate derived nontumorigenic or tumor cell lines by Western blotting. The bar graph on the right shows quantitative data from three independent blots. ∗ p < 0.05 by two-way ANOVA. Prx, peroxiredoxin.

Article Snippet: Human prostate normal and prostate cancer tissue microarray slides were commercially obtained, including BNS19011 and PR803d (US Biomax).

Techniques: Microarray, Western Blot, Staining, Derivative Assay

Journal: Molecular cancer research : MCR

Article Title: DNA Damage Induces a Secretory Program in the Quiescent TME that Fosters Adverse Cancer Phenotypes

doi: 10.1158/1541-7786.MCR-16-0387

Figure Lengend Snippet: a Quantitation of gene expression in microdissected prostate stroma by microarray hybridization before (Pre) and after (Post) exposure to mitoxantrone and docetaxel (≥1.5-fold increase with p<0.05). Expanded heatmap shows a subset of the transcripts encoding extracellular proteins.

Article Snippet: Prostate cancer tissue microarray slides were scanned on Aperio ScanScope AT (Aperio Technologies, Vista, CA, USA).

Techniques: Quantitation Assay, Gene Expression, Microarray, Hybridization

Journal: Carcinogenesis

Article Title: Muscadine grape skin extract can antagonize Snail-cathepsin L-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells

doi: 10.1093/carcin/bgv084

Figure Lengend Snippet: CatL expression increases with tumor progression. (A) Immunohistochemical (IHC) analysis was performed using a 96 core prostate adenocarcinoma tissue microarray. Representive images of CatL in various stages of prostate cancer show that CatL increases with tumor progression. Bar represents 50 µM. (B) Normal/tumor matched invasive ductal carcinoma (IDC) grades 1 and 3, infiltrating carcinoma (IFC) grade 3, adenocarcinoma (grade 3) and metaplastic carcinoma (MPC) grade 3 were analyzed for expression of CatL by western blot analysis. Mature CatL expression was generally higher in tumor as compared to normal tissue. Alpha (α)-tubulin was used as a loading control. Data are representative of at least three independent experiments.

Article Snippet: Immunohistochemistry Examination of the expression and distribution of CatL in human prostate cancer was performed by immunohistochemistry (IHC) using prostate normal and cancer tissue microarray slides obtained from US Biomax (PR956a, Rockville, MD).

Techniques: Expressing, Immunohistochemical staining, Microarray, Western Blot